E. coli Plasmid Vectors: Methods and Applications

The Function And Organization Of Plasmids / Finbar Hayes -- Choosing A Cloning Vector / Andrew Preston -- Escherichia Coli Host Strains / Nicola Casali -- Chemical Transformation Of E. Coli / W. Edward Swords -- Electroporation Of E. Coli / Claire A. Woodall -- Dna Transfer By Bacterial Conjugation / Claire A. Woodall -- Cosmid Packaging And Infection Of E. Coli / Mallory J.a. White -- Isolation Of Plasmids From E. Coli By Alkaline Lysis / Sabine Ehrt -- Isolation Of Plasmids From E. Coli By Boiling Lysis / Sabine Ehrt -- High-purity Plasmid Isolation Using Silica Oxide / Stefan Grimm -- High-throughput Plasmid Extraction Using Microtiter Plates / Michael A. Quail -- Isolation Of Cosmid And Bac Dna From E. Coli / Daniel Sinnett -- Preparation Of Single-stranded Dna From Phagemid Vectors / W. Edward Swords -- Using Desktop Cloning Software To Plan, Track, And Evaluate Cloning Projects / Robert H. Gross -- Cloning In Plasmid Vectors / Carey Pashley --^ Extraction Of Dna From Agarose Gels / Nicholas Downey -- Cloning Pcr Products With T-vectors / Wade A. Nichols -- Construction Of Genomic Libraries In V-vectors / Yilun Wang -- Rapid Screening Of Recombinant Plasmids / Sangwei Lu -- Restriction Analysis Of Recombinant Plasmids / Joanne Goranson-siekierke -- Screening Recombinant Dna Libraries / Wade A. Nichols -- Sequencing Using Fluorescent-labeled Nucleotides / Allison F. Gillaspy -- Site-directed Mutagenesis Using The Megaprimer Method / Zhidong Xu -- Site-directed Mutagenesis By Inverse Pcr / Clifford N. Dominy -- Creating Nested Dna Deletions Using Exonuclease Iii / Rosamund Powles -- Transposon And Transposome Mutagenesis Of Plasmids, Cosmids, And Bacs / Alistair Mcgregor -- In Vitro Transcription And Translation / Farahnaz Movahedzadeh -- Vectors For The Expression Of Recombinant Proteins In E. Coli / Sally A. Cantrell -- Expression Of Recombinant Proteins From Lac Promoters / Charles R. Sweet --^ Plasmid-based Reporter Genes: Assays For B-galactosidase And Alkaline Phosphatase Activites / Minghsun Liu -- Plasmid-based Reporter Genes: Assays For Green Fluorescent Protein / Sergei R. Doulatov. Edited By Nicola Casali And Andrew Preston. Includes Bibliographical References And Index.