Peptide Chemistry: A Practical Textbook

Structure Determination -- Amino Acid Analysis -- Sequence Determination -- End Group Determination -- Sequential Degradation -- Sequence Determination With The Help Of Mass Spectra -- Fragmentation Of Peptides -- Secondary And Tertiary Structure -- Architectural Features In Peptides -- Methods For The Analysis Of Conformation Of Peptides -- Prediction Of Conformation In Peptides -- Peptide Synthesis -- Formation Of The Peptide Bond -- Acid Chloride Method -- Acid Azide Method -- Anhydrides -- Active Esters -- Coupling Reagents -- Enzyme-catalyzed Bond Formation -- Non-conventional Methods Of Peptide Bond Formation -- Protection Of Functional Groups -- Blocking Of α-amino Groups -- Blocking Of Side Chain Amino Groups -- Protection Of The Carboxyl Group -- Protection Of Hydroxyl Groups -- Blocking Of The Sulfhydryl Group -- Masking The Thioether In Methionine -- Protection Of The Guanidine Function In The Arginine Side Chain -- Masking The Imidazole In Histidine -- Indole Protection -- Carboxamide Blocking Groups. (cont) Undesired Reactions During Synthesis -- Base-catalyzed Ring Closure -- Acid-catalyzed Ring Closure -- Acyl Migration -- Alkylation -- Friedel-crafts Reaction -- Saturation Of Aromatic Nuclei -- Reductive Cleavage Of The Peptide Chain -- Oxidative Decomposition Of The Indole In Tryptophan -- Side Reactions Caused By Steric Hindrance -- Prevention And Suppression Of Side Reactions -- Racemization -- Mechanism Of Racemization -- Detection Of Racemization -- Racemization Studies In Model Systems -- Prevention Of Racemization -- Design Of Schemes For Peptide Synthesis -- Segment Condensation -- Stepwise Chain-lengthening Starting With The N-terminal Residue -- Stepwise Chain-lengthening Starting With The C-terminal Residue -- Tactical Consideration -- Synthesis Of Special Target Peptides -- Solid Phase Peptide Synthesis -- Insoluble Polymeric Support And The Bond Linking The Peptide To The Resin -- Removal Of Temporary Blocking Groups -- Coupling: Acylation Of The Resin-bound Amine-component -- Separation Of The Completed Peptide Chain From The Polymeric Support And Final Deprotection. (cont) Some Problems Encountered In Solid Phase Peptide Synthesis -- Methods Of Facilitation -- Chains Attached To A Soluble Polymer -- Picolyl Ester Method -- Rapid Synthesis -- Synthesis In Situ -- Simultaneous Synthesis Of Peptide Analogs -- Insoluble Reagents -- Analysis And Characterization Of Synthetic Peptides -- Homogeneity Tests -- Amino Acid Analysis And Sequence Determination -- Elemental Analysis -- Measurement Of Optical Activity -- Infrared And Ultraviolet Absorption Spectra -- Nuclear Magnetic Resonance Spectra -- Molecular-weight Determination. Miklos Bodanszky. Includes Bibliographical References And Index.